New pacidamycins biosynthetically: probing N- and C-terminal substrate specificity

Amany E. Ragab ; Sabine Grüschow ; Emma J. Rackham ; Rebecca J. M. Goss Org Biomol Chem 2010, 8 3128-3129 Feeding phenylalanine analogues to Streptomyces coeruleorubidus reveals the remarkable steric and electronic flexibility of this biosynthetic pathway and leads to the generation of a series of new halopacidamycins.

A Serine Carboxypeptidase-Like Acyltransferase Is Required for Synthesis of Antimicrobial Compounds and Disease Resistance in Oats

Sam T. Mugford ; Xiaoquan Qi ; Saleha Bakht ; Lionel Hill ; Eva Wegel ; Richard K. Hughes ; Kalliopi Papadopoulou ; Rachel Melton ; Mark Philo ; Frank Sainsbury ; George P. Lomonossoff ; Abhijeet Deb Roy ; Rebecca J. M. Goss ; Anne Osbourn The Plant Cell 2009, 21 (8) 2473-2484 Serine carboxypeptidase-like (SCPL) … Read more

New Pacidamycin Antibiotics Through Precursor-Directed Biosynthesis

Sabine Gruschow ; Emma J. Rackham ; Benjamin Elkins ; Philip L. A. Newilll ; Lionel M. Hill ; Rebecca J. M. Goss ChemBioChem 2009, 10 (2) 355-360 Pacidamycins, mureidomycins and napsamycins are structurally related uridyl peptide antibiotics that inhibit translocase I, an as yet clinically unexploited target. This potentially important bioactivity coupled to the biosynthetically intriguing … Read more

A convenient one-step synthesis of L-aminotryptophans and improved synthesis of 5-fluorotryptophan

Michael Winn ; Abhijeet Deb Roy ; Sabine Grüschow ; Raj S. Parameswaran ; Rebecca J. M. Goss Bioorg Med Chem Lett 2008, 18 (16) 4508-4510 A one-pot biotransformation for the generation of a series of l-aminotryptophans using a readily prepared protein extract containing tryptophan synthase is reported. The extract exhibits remarkable stability upon freeze-drying, and may … Read more

Generating rapamycin analogues by directed biosynthesis: starter acid substrate specificity of mono-substituted cyclohexane carboxylic acids

Rebecca J. M. Goss ; Simon E. Lanceron ; Nicola J. Wise ; Steven J. Moss Org Biomol Chem 2006, 4, 4071-4073 We report a convenient synthesis of 4-fluorocyclohexanoic acid, and an insight into the rules governing acceptance of starter acid analogues in the precursor-directed biosynthesis of rapamycin.