Abhijeet Deb Roy ; Sabine Grueschow ; Nickiwe Cairns ; Rebecca J. M. Goss
JACS 2010, 132 (35) 12243-12245
Introduction of prnA, the halogenase gene from pyrrolnitrin biosynthesis, into Streptomyces coeruleorubidus resulted in efficient in situ chlorination of the uridyl peptide antibotic pacidamycin. The installed chlorine provided a selectably functionalizable handle enabling synthetic modification of the natural product using mild cross-coupling conditions in crude aqueous extracts of the culture broth.
