Characterisation of spin coated engineered Escherichia coli biofilms using atomic force microscopy

Andreas N. Tsoligkas ; James Bowen ; Michael Winn ; Rebecca J. M. Goss ; Tim W. Overton ; Mark J. H. Simmons Colloids and Surfaces B: Biointerfaces 2012, 89 152-160 The ability of biofilms to withstand chemical and physical extremes gives them the potential to be developed as robust biocatalysts. Critical to this issue is their … Read more

Biofilms and their engineered counterparts: A new generation of immobilised biocatalysts

Michael Winn ; Joanne M. Foulkes ; Stefano Perni ; Mark J. H. Simmons ; Tim W. Overton ; Rebecca J. M. Goss Cat Sci Tech 2012, 2 1544-1547 The robust nature of biofilms makes them medicinally difficult to treat, however this same property renders them an attractive method for protecting and immobilising enzymes for biotransformation. … Read more

The generation of “unNatural” products: Synthetic biology meets synthetic chemistry

Rebecca J. M. Goss ; Sreejith Shankar ; Antoine Abou Fayad Nat Prod Rep 2012, 29 870-889 Natural product analogue generation is important, providing tools for chemical biology, enabling structure activity relationship determination and insight into the way in which natural products interact with their target biomolecules. The generation of analogues is also often necessary in … Read more

Biogenesis of the Unique 4 ‘,5 ‘-Dehydronucleoside of the Uridyl Peptide Antibiotic Pacidamycin

Amany E. Ragab ; Sabine Grüschow ; Daniel R. Tromans ; Rebecca J. M. Goss JACS 2011, 133 (39) 15288-15291 The pacidamycins belong to a class of antimicrobial nucleoside antibiotics that act by inhibiting the clinically unexploited target translocase I, a key enzyme in peptidoglycan assembly. As with other nucleoside antibiotics, the pacidamycin 4′,5′-dehydronucleoside portion is … Read more

A Single Streptomyces Symbiont Makes Multiple Antifungals to Support the Fungus Farming Ant Acromyrmex octospinosus

Ryan F. Seipke ; Joerg Barke ; Charles Brearley ; Lionel Hill ; Douglas W. Yu ; Rebecca J. M. Goss ; Matthew I. Hutchings PLos ONE 2011, 6 (8) e22028 Attine ants are dependent on a cultivated fungus for food and use antibiotics produced by symbiotic Actinobacteria as weedkillers in their fungus gardens. Actinobacterial species belonging … Read more

Engineering Biofilms for Biocatalysis

Andreas N. Tsoligkas ; Michael Winn ; James Bowen ; Tim W. Overton ; Mark J. H. Simmons ; Rebecca J. M. Goss ChemBioChem 2011, 12 (9) 1391-1395 Biofilm, friend not foe: Single species biofilms can be engineered to form robust biocatalysts with greater catalytic activity and significantly improved catalytic longevity than purified and immobilised enzymes. We … Read more

Diversity in natural product families is governed by more than enzyme promiscuity alone : establishing control of the pacidamycin portfolio

Sabine Grüschow ; Emma J. Rackham ; Rebecca J. M. Goss Chem Sci 2011, 2 2182-2186 As with many other antibiotics, pacidamycins are produced as a suite of related compounds. Unlike most other secondary metabolites, however, this diversity is not solely the result of the substrate promiscuity of the biosynthetic enzymes but also arises from a gene duplication event (Pac21, … Read more

The saponins – polar isoprenoids with important and diverse biological activities

Anne Osbourn ; Rebecca J. M. Goss ; Robert A. Field Nat Prod Rep 2011, 28 1261-1268 Saponins are polar molecules that consist of a triterpene or steroid aglycone with one or more sugar chains. They are one of the most numerous and diverse groups of plant natural products. These molecules have important ecological and agronomic functions, contributing to … Read more

Gene Expression Enabling Synthetic Diversification of Natural Products : Chemogenetic Generation of Pacidamycin Analogs

Abhijeet Deb Roy ; Sabine Grueschow ; Nickiwe Cairns ; Rebecca J. M. Goss JACS 2010, 132 (35) 12243-12245 Introduction of prnA, the halogenase gene from pyrrolnitrin biosynthesis, into Streptomyces coeruleorubidus resulted in efficient in situ chlorination of the uridyl peptide antibotic pacidamycin. The installed chlorine provided a selectably functionalizable handle enabling synthetic modification of the natural product using mild cross-coupling … Read more